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          BMPFs.ÉϵÄÆäËüÌDzлù,ÀýÈç°¢À­²®ÌÇ»á¶ÔÆä»îÐÔ±¬·¢Ó°Ïì¡£Gao(Gao,et al,2013)ÊӲ쵽°¢À­²®ÌǼȿÉÒÔÔö¼Ó¡¢Ò²¿ÉÒÔ¼õÉÙ°ëÈé¹Ñ¾ÛÌÇƬ¶ÎÓëGal3¼äµÄÏ໥×÷Óá£ÓеÄÄý¼¯ËØÆäÅä»ùµÄµ¹ÊýµÚ¶þ¸öÌDzлù»áÓ°ÏìÆäʶ±ðר-ÐÔ(Nakahara,&Raz,A.2008).¡£Ä¿Ç°ÉÐδÓÐÑо¿BMPFsÆäËûÄ©¶ËÌDzлù¶ÔÆäר-ÐÔÓ°ÏìµÄ±¨µÀ¡£ÓÉÓÚÌåÄÚÓÐÊ®¼¸ÖÖ°ëÈéÌÇÄý¼¯ËØ£¬Òò¶ø£¬¶ÔÅäÌåÄ©¶ËÅâ²Ð»ùµÄ½á¹¹Ñо¿ÊÇÐëÒªµÄ¡£

          Gal-3Óë¶àÖÖ¼²²¡ÓйØ£¬BMPFsÈçºÎÓ°ÏìGal-3Ïà¹ØµÄ¼²²¡£¬Ñо¿Àý×Óδ¼¸¡£Ò»¸öÓйصÄÀý×ÓÊÇ£¬MCP¶ÔСÊóʵÑéÐÔÉöËðÉËÓб£»¤×÷Óã¬Õâ¿ÉÄÜÓëMCPµ÷µÍ

      Gal-3µÄ±í´ïˮƽÓйØ£¬Í¬Ê±MCP²»Ó°ÏìGal-1 ºÍGal-9µÄ±í´ïˮƽ(Kolatsi-Joannou, Price, Winyard, & Long, 2011)¡£ÌåÄÚ±£´æÆäËü»îÐÔ¸÷ÒìµÄ°ëÈéÌÇÄý¼¯ËØ(ÖÁÉÙÓÐÊ®ÎåÖÖ),ËüÃǾùʶ±ð¾ßÓаëÈéÌÇδ¶ËµÄ·Ö×Ó£¬Òò¶ø²»¿ÉÅųýBMPFsÓëGal-3 Ö®ÍâµÄ°ëÈéÌÇÄý¼¯Ëر¬·¢Ï໥×÷ÓõĿÉÄÜÐÔ(Heusschen,Grifioen, & Thissen, 2013)¡£BMPFs ÆäÌåÄڵİеã³ýGal-3Í⣬ÊÇ·ñ»¹Äܹ»ÓëÆäËû¼Ò×åµÄ°ëÈéÌÇÄý¼¯ËØ(ÀýÈçASGP-R)¡¢Ï¸°ûÒò×Ó(Liu et al, 2001; Salman,Bergman, Djaleti, Orlin, & Bessler, 2008)¡¢ËÀÍöÊÜÌå(Chauhan et al, 2005)Ï໥×÷Óã¬Óдý½øÒ»²½Ñо¿¡£

          6.¹û½ºÔÚ¿¹°©¸øҩϵͳÖеÄÓ¦ÓÃ

          ¹û½ºÊÇÒ»ÖÖÐí¶à¹ú¼ÒÅú׼ʹÓõÄÒ©Î︨ÁÏ¡£ÓÉÓÚËü¾ßÓÐôÇ»ù¡¢ôÈ»ùµÈ¹¦Ð§»ùÍÅ£¬Ò×ÓÚÐÞÊΣ¬ÎÞ¶¾ÐÔ£¬Òò¶øÔÚ¸øҩϵͳÖÐÓ¦Óù㷺¡£Æ¾¾Ý¸øҩ;¾¶£¬¹û½º¸øҩϵͳ·ÖΪ賦µÀ(¾­¿Ú·þ)¸øÒ©ºÍ·Ç賦µÀ¸øÒ©¡£Î¸³¦µÀ¸øÒ©µÄÖØÒª·½·¨Êǽ᳦¸øҩϵͳ(colon-specific drug delivery system, CSDDS),½ÓÄÉ¿Ú·þ¸øÒ©£¬ÄÜÔö¼Ó²¡È˽ÓÊÜÖÎÁƵÄ˳ӦÐÔ(Dev, Bali, & Pathak, 2011; Wong, Colombo, & Sonvico,2011)¡£ÀûÓý᳦ÄÚϸ¾úÊͷŵÄø¿ÉÒÔ½µ½â¹û½ºµ¼ÖÂÊÍÒ©µÄÔ­ÀíÒÔ¼°¶Ô¹û½º½øÐÐÄý½º»¯¡¢¸Æ»¯¡¢¾ÛºÏ³Éй²¾ÛÎïµÄÒªÁì¿ÉÒÔÖƱ¸CSDDS, CSDDSÄÜÔöǿijЩҩÎïµÄÉúÎïÀûÓöÈ¡£Ò©´ú¶¯Á¦Ñ§ÊµÑéÖ¤Ã÷£¬»ùÓÚ¹û½ºµÄCSDDS¾ßÓÐÁ¼ºÃµÄ¾Ö²¿ÊÍÒ©ÄÜÁ¦£¬ÓÐÀûÓÚÖÎÁƽ᳦°©(He, Du, Cao, Xiang, & Fan, 2008)¡£·Ç賦µÀ¸øÒ©ÐèҪʹÓøÄÐԵĹû½º¡£ÀýÈ磬- -ÖÖÒÔõ£°·»¯¹û½ºÎªÔØÌåµÄDNAÔØÔËϵͳ£¬Ê¹ÓÃÁË´øÓÐÕýµãµçºÉµÄ¹û½º¸ÄÐÔ·Ö×Ó£¬Äܹ»Ê¹ÍâÔ´»ùÒòת»¯½øÈëHEK293ϸ°ûÄÚ(Katavet al.2008)¡£µÍõ¥¹û½º(·Ö×ÓÁ¿< 50 00Da)Ó밢ùËØéîºÏµÄ¸øҩϵͳ£¬¾­ÊóÄ£ÐÍÌåÄÚʵÑéÖ¤Ã÷£¬ÄÜÒÖÖÆÐþÉ«ËØÁöתÒÆ(Tang et al, 2010)¡£¹û½ºµÄRG-IƬ¶Î¸»º¬Ä©¶Ë°ëÈéÌÇ£¬¶ø¸ÎÔàÍâòµÄÒ»ÖÖ°ëÈéÌÇÄý¼¯ËØ- -ASGP-R »òÐíÄܹ»Ê¶±ðÕâÀàÌDzлù¡£Òò¶ø£¬ÀûÓùû½º»òÕßMCPÄÜ¿ª·¢Õë¶Ô¸Î°©µÄÆ÷¹Ù°ÐÏò¸øҩϵͳ(Yuetal,2014)¡£

          ±ðµÄ£¬¹û½º¿ÉÒÔÖ±½Ó¾­ÐÞÊÎÓ뿹Ö×ÁöÒ©Îï½øÐл¯Ñ§½»ÁªÉè¼Æǰҩϵͳ£¬»òÖƱ¸³ÉË®Äý½º¡¢Î¢ÇòµÈÖÖÖÖÐÎʽµÄ¿¹°©Ò©ÎﻺÊÍϵͳ(Puga, Lima, Mano, Concheiro,& Alvarez-Lorenzo, 2013; Takei, Sato, ljima, & Kawakami, 2010)£¬ÕâЩ¸øҩϵͳÓÐ

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          ±¾ÎĶÔDF-Pec¡¢BMPFs¼°¹û½º¸øҩϵͳÔÚ¿¹Ö×ÁöÖÎÁÆ·½ÃæµÄÓ¦ÓýøÐÐÁË×ܽᣬ²¢Öصã¶ÔBMPFsµÄ¿¹Ö×Áö»úÖƺ͹¹Ð§¹Øϵ½øÐÐÁËÏêϸÆÊÎö¡£ÌìÈ»¹û½º½á¹¹ÅӴ󡢿¹Ö×Áö¹û½º¹¦Ð§Ê³Æ·¹æ¸ñ·×Æ磬²»¹ý´ó²¿·Ö±¨¸æÖ§³ÖDF-PecÄÜ·ÀÖν᳦°©£¬Æä»úÖÆÉæ¼°ÒÖÖÆ·¢°©ºÍÃâÒßµ÷Àí¡£Ï¸ÖÂÀí½âDF-Pec µÄ¿¹Ö×Áö¹¹Ð§¹ØϵÓÐÖúÓÚ¸ïвúÆ·¹¦Ð§£¬ÒÔ»ñµÃ½á¹¹ºÍ¹¤ÒÕ¸ü- -Ö¡¢¿É¿ØµÄ²úÆ·£¬ÓÐÖúÓÚ»ñµÃЧ¹û¸ü¼ÑµÄ»ùÓÚ¹û½ºµÄ±£½¡Æ·(nutraceutical)(McCarty & Block, 2006)¡£×ÜÌå¶øÑÔ£¬½«¹û½º¼°Æä¸ÄÐÔÎ↑·¢Îª¿¹°©Ò©ÎïµÄÔØÒ©ÖÊÁÏËùÃæÁÙµÄÌôÕ½ÉÙÓÚ½«BMPFs¿ª·¢³ÉΪ¿¹Ö×ÁöÒ©ÎïµÄÀú³Ì¡£

          һЩ»ú¹¹¿ªÊ¼×ÅÊÖ½«BMPFs¿ª·¢³ÉÒ©Îµ«»¹±£´æÐí¶àÎÊÌâÓдý½â¾ö¡£ÀýÈ磬¹û½º½á¹¹ÈçºÎÊܼӹ¤Àú³ÌºÍ³¦µÀ¾úȺµÄÓ°Ïì;¼ÁÐͺ͹û½ºµÄÄý½º»¯ÈçºÎÓ°ÏìÆäÉúÎïÀûÓöÈ;½ÓÄÉÔõÑùµÄ±ê×¼ÓÅ»¯¸ÄÐÔÀú³Ì;ÈçºÎ¿ØÖÆÆä·Ö×ÓÁ¿ÂþÑÜ( Zhang, etal, 2010);ÈçºÎÍêÉÆÖÊÁ¿¿ØÖƼƻ®;ÔÚÌåÄÚÁíÓÐÄÄЩÒòËØÓ°ÏìBMPFsÓë°Ðµã¼äµÄÏ໥×÷ÓÃ;BMPFsÔÚ¿¹Ö×Áöʧ³²µòÍö·½ÃæÔÚ°ûÄÚÆð×÷ÓÃÕÕ¾ÉÔÚ°ûÍâÆð×÷ÓÃ;BMPFsÔÚÌåÄÚÊÇ·ñ»áÕûºÏ²¬ÀàÒ©Îï?ÈËÌåÄܹ»±¬·¢Ò»ÖÖÕë¶ÔÊóÀîÌǵĿ¹Ìå(Pazur, Erikson, Tay, & Alen, 1983)¡£Èç¹ûBMPFs±£´æÉÙÁ¿Ä©¶ËÊóÀîÌÇÌÇÜÕ£¬ºã¾ÃÖظ´Ê¹ÓÃBMPFsÊÇ·ñ»áÓÕµ¼ÌåÄÚ±¬·¢Çå³ýËüÃǵĿ¹Ìå?BMPFs¿ÉÒÔÖÎÁÆGal-3Ïà¹ØµÄÆäËû¼²²¡(KolatsiJoannou, 2011),¶ÔÕâЩ¼²²¡µÄÖÎÁÆ»á¶ÔÖ×ÁöÖÎÁƱ¬·¢Ê²Ã´Ó°Ïì?½øÒ»Ò»²½µÄÊÂÇéÄܹ»¸ÄÉÆBMPFsµÄ»îÐÔ»òÕßÀ©Õ¹ÆäÓ¦ÓÃÁìÓò¡£ÀýÈ磬ÊÇ·ñ¿ÉÒÔͨ¹ý½«BMPFs½øÒ»²½ÐÞÊλñµÃÉúÎïÀûÓöȻòÇ׺ÍÁ¦¸üºÃµÄƬ¶Î?ÓëÎüÊÕÔö½ø¼ÁÅäºÏ¿É·ñÔö¼ÓÆäÉúÎïÀûÓöÈ?ÄÜ·ñ½«BMPFséîºÏÓ«¹â·Ö×Ó±ê¼Ç»òÕßÁ¿×ӵ㣬³ÉΪGal-3¡¢Ñ­»·Ö×Áöϸ°û»òÕß΢תÒƵļì²âÊÔ¼Á¡¢Ê¾×Ù¼Á? BMPFsÖ±½ÓÕվɼä½ÓÓ°Ïìϸ°ûÒò×ӵĹ¦Ð§(Dennis, Lau, Demetriou, & Nabi, 2009) ?Õë¶ÔÈç´ËÖ®¶àµÄÎÊÌ⣬×÷ÕߵĽ¨ÒéÊÇÓ¦ÖصãÉîÈëÑо¿Gal-3µÄ¹¦Ð§Óë±í´ïÇé¿ö¡¢Æ÷¹ÙÂþÑÜ¡¢BMPFs µÄ¹¹Ð§¹Øϵ£¬»ñµÃ¸ü¶àµÄҩЧѧ¡¢Ò©´úѧÒÔ¼°ÁÙ´²Ñо¿Êý¾Ý¡£

    Acknowledgement

          This work was supported by the Guide Project of Science and TechnologyResearch of Henan Education Department (12B350006). Authors would like todeclare no conflicts of interest.

    Abbreviations

    AG-I, type I arabinogalactan; AG-II, type I arabinogalactan; ASGP-R,asialoglycoprotein receptor; BMPF, Bioavailable modified pectin fragment; CP, citruspectin; CRD, carbohydrate recognition domain; CSDDS, colon- specific drug deliverysystem; DF, dietary fiber; DF-Pec, dietary fiber pectin; DM, degree of methylation;ECM, extracellular matrix; Gal-3, galectin-3; Gal-3l, galectin-3 inhibitor; GALT,gut-associated lymphoid  tissue;  GS, substituted  galacturonans;  HG,homogalacturonan; HTCP, heat-treated citrus pectin; MCP, modified citrus pectin;PUFA, polyunsaturated fatty  acids; RG-I, rhamnogalacturonan-I; RG-II,rhamnogalacturonan-I; SCFA, short-chain fatty acids; XGA, xylogalacturonan.

    References.

          Attari, F., Sepehri, H., Delphi, L., & Goliaei, B. (2009). Apoptotic and necroticeffects of pectic acid on rat pituitary GH3/B6 tumor cells. Iranian Biomedical Journal,13(4):229-36.

          Az¨¦mar, M., Hildenbrand, B., Haering, B., Heim M.E. & Unger, c. (2007).Clinical benefit in patients with advanced solid tumors treated with Modified CitrusPectin: a prospective pilot study. Clinical Medicine: Oncology, 1:73. -80.

          Avivi-Green, c., Polak-Charcon, s.. Madar, Z., & Schwartz, B. (2000a).Apoptosis cascade proteins are regulated in vivo by high intracolonicbutyrate concentration: correlation with colon cancer  inhibition. OncologyResearch, 12(2): 83-95.

          Avivi-Green, c.. Polak-Charcon, S., Madar, Z, & Schwartz, B. (2000b). Dietary

     Application of Pectin in Cancer Therapy: a Review

          Wenbo Zhang". Han Zhang", Ping Xu*

          (a. School of Life Science and Technology, Xinxiang Medical University, XinxiangHenan 453003, PR China; b. School of Pharmacy, Xinxiang Medical University,

          Xinxiang Henan 453003, PR China)

          Key words: cancer therapy, galectin-3, modified citrus pectin, pectin



     



     



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